Luminex may be a misleading term. The actual assay refers to a bead based multiplexing technique which in large part was developed right here at RPCI under the leadership of Carleton Stewart. The Luminex Corp. is the leading company that has commercialized various applications of this technique.

The basis of the Luminex assay is the xMAP (any Multiple Analyte Profiling) technology which color-codes microspheres with different combinations of 10 shades of 2 dyes creating a 100 bead sets that can be discriminated by flow cytometry. Each bead can be coated with an analyte specific capture reagent (antibody) and binding of the reagent can then be quantified using reporter antibodies. Thus, xMAP technology allows multiplexing of up to 100 unique assays within a single sample. The Luminex-100 instrument has 2 lasers in which one is used to excite and detect a specific bead and the other to detect the PE bound to a reporter antibody.

beads

combination of two dyes with ten different concentrations will combine for 100 unique beads

multiplex sandwich

beads labeled
with unique
captive antibody

Luminex

detection
of antigen

In our facility, the assay is performed in a 96-well plate format of which commonly 80 wells are available for sample analysis (16 wells are used for internal standards and controls). Thus in principle, 100 analytes can be assayed on 80 samples in a single experiment.

Common applications: protein expression profiling (detection of cytokines, chemokines, phospho-proteins, cell signaling, isotyping, apoptosis, etc. in serum, plasma, cell culture supernatant, cell lysate). For a full repertoire of available analytes please see the websites of our preferred vendors. Links top these web sites are provided on the right.

Actual costs for the assay are dependent on a number of variables including the number of samples, the number and specific characteristics of the analytes you are interested in and the level of service requested. For a detailed estimate on your specific study, please contact us.

Links for Service Request Forms, accepted and preferred sample preparation, available services and associated rates are provided on the left.

The latest applications of the soluble bead array technology are molecular-based assays in which the beads are coated with nucleic-acid capture sequences rather than antibodies. Applications of this multiplexing approach are in the field of SNP detection and miRNA detection. If you have specific interest in these applications please see the vendors' web sites or contact the facility.

Luminex facility contacts:

Luminex Assay Kit Manufacturers

Areas of Research and Available Analytes