Clinical Flow Cytometry Services

Our facility maintains a CLIA-certified laboratory that provides processing and flow cytometry analysis services for diagnostic patient samples and clinical experimental protocols. The lab receives samples from all over the nation. For available tests, click here.

Flow Cytometry Resource Facility

Our Research Flow Cytometry facility houses 5 flow analyzers that are accessible 24 hours a day, 7 days a week to all investigators, as well as 2 cell sorters and several breadboard instruments that are operator-assisted and available during regular weekday business hours (8:00 A.M. to 6:00 P.M. Monday – Friday).

Flow Analyzers

In the 24/7 accessible user room we currently have:

During weekdays, staff will perform start-up procedures and daily QC on these instruments. Users can sign up for time on the equipment using a web-based calendar system. For new user accounts, please contact us with the required information. Principal investigators are billed for booked calendar time and usage time at the end of each month. For more information on billing policies, click here.

In our experience, there are three types of research investigators who use the Flow Cytometry Resource:

Cell Sorters

The Flow Sorting Facility houses two cell sorter instruments, FACSAria I and FACSAria II. The FACSAria I was upgraded in September 2010 and is equipped with 18 fluorescent PMT's and 405, 488, 561, and 640 nm lasers, and has bio-containment capabilities. The FACSAria II is equipped with four lasers (355 nm, 408 nm, 488 nm, and 633 nm) and has 13 fluorescent detectors. Both instruments have the capacity to do four-way sorts as well as sorting into microtiter plates (96-, 48-, 24-, 12-, or 6-wells) and onto slides. The instruments are operated by facility staff members and available during regular business hours by arrangement only. For more information consult the Sorting facility website.

Breadboard Flow Cytometers

The Flow and Imaging Electronics Shop provides a unique service to the core facility. The modestly equipped shop has the ability to service, repair modify or construct flow cytometers utilized by Roswell staff. Custom technology available for studies includes contiguous multispectral detection using diffraction grating optics, Infra Red flow cytometry, fluorescence detection beyond 1100 nm and an optical Breadboard flow cytometer open to diverse configurations. Researchers interested in using this technology are to contact Ed Posniesinski (x8775).

DEPARTMENT OF FLOW
AND IMAGE CYTOMETRY

Roswell Park Cancer Institute
Elm and Carlton Streets
Buffalo, New York 14263

Main Phone: 716.845.8471
Clinical Phone: 716.845.3528
Fax: 716.845.8806

Paul K. Wallace, Director
Hans Minderman, Co-Director

 

FluoroFinder provides links that enable you to view configurations for each flow cytometer and to begin building a panel or enter a panel that has already been designed.