Paul Wallace Dr Paul Wallace Ph.D.
Paul is the leader of this band of Flow Cytometrists. He graduated from New England College with a Bachelor of Science degree in 1975. He then received his Masters in Immunology from Idaho State University in 1979 and his Doctorate in Immulnology from the Medical College of Pennsylvania in 1992. Paul was a supervisor of Microbiology and Immunology at Smith Kline Clinical Labs (now Quest Labs) where he pioneered the development of Clinical Flow Cytometry. In 1993, he moved to New Hampshire where he did Post Doctoral work at Dartmouth Medical School and subsequently served as an assistant professor while investigating the use of biospecific antibodies and fusion proteins targeting CD64 as tumor vaccines and mediators of cytotoxicity. Paul joined Roswell Park in 2003 where he oversees the day to day operation of the Clinical and Research Flow Cytometry Labs.
Hans Minderman Hans Minderman, Ph.D.
Hans Minderman is the Assistant Director of the Flow and Image Cytometry. Together with Paul Wallace he oversees the daily research activities in the facilities in particular with regards to imaging. Hans received his bachelors degree in Medical Biology from the Free University of Amsterdam, the Netherlands, and received his PhD from the University of Nijmegen, the Netherlands, where he was first introduced to Flow Cytometry working on his thesis on "Anthracycline Toxicity in Relation to the Proliferative State of the Cell". This was at a time when Flow Cytometry data was still captured on punch cards (!). Part of Hans' thesis research was conducted in collaboration at RPCI which brought him to Buffalo which he thought was in Texas. The plan was to conduct studies for only six weeks but twenty-one years later he's still here! Hans' own research interest is focused on quantifying the activity of transcription factors (eg. NFkB, NFAT) in immunophenotypically-defined cell populations as a parameter of response to therapies targeting these pathways using the ImageStream technology. These NIH-funded studies are conducted in collaborations with research groups inside and outside of RPCI as well as pharmaceutical industry. The work with the ImageStream platform has earned our facility international recognition for being at the cutting edge of clinical application of this technology and we regularly host training sessions for national and international collaborators.
Kieran O'Loughlin Kieran O'Loughlin, M.S.
Kieran O'Loughlin (BS) has ten years of Flow Cytometry and translational research experience. He has contributed his expertise to various peer reviewed grant applications and publications. He has also been involved with training of medical fellows and students of all levels on specific laboratory techniques, including flow cytometry. Mr. O'Loughlin assists facility users with experimental planning, data acquisition and analysis for the ImageSteam technology, Flow Cytometry (FC500, FacsCalibur, FacsAria) and Live Cell Imaging.
Ed Podniesinski Ed Podniesinski, Flow Engineer
Ed was hired in 1991 to bring to fruition the ideas and concepts of the previous department director Dr. Carleton Stewart through cytometer modifications. At that time Flow Cytometry was an experimental up and coming technology and his abilities contributed to the department's growth. Today he continues with the current director Dr. Paul Wallace. Besides this fun aspect of his job he also provides service support for all of our Core facility Research/Clinical cytometers (analyzers and sorters) with a substantial cost saving as we carry no service agreements. He has developed a preventive maintenance program for all instruments, which has significantly reduced maintenance costs. He manages all data processing hardware and interfaces with the Information Technology Department to maintain our network with the rest of the Institute. Because of his unique expertise, Ed also provides some of these services to the Institute as a whole.
Rose Furlage Rose Furlage
Rose started at RPCI in 2004. Her Flow experience began in 1990 when she was trained on the FACSStar and then moved to the FACSScan and the FACSCaliber. She received a Qualification in Cytometry from ASCP in 1997. Since arriving here, Rose has done both clinical and research samples and currently is focusing on research. She has been trained on the FACSCanto, LSR and Aria. She has a BS in Medical Technology and has been working in this field since 1972 with the exception of a few years spent working for the IRS, (what was she thinking?!).
Alixis Conway Alexis Conway
Alexis Conway is a Research Technologist and has been working in the Flow Cytometry Department since March of 2006 and holds a Bachelor's Degree in Biology from Alderson-Broaddus College. She is the primary liaison between the Flow lab and our client Immune Tolerance Network. Alexis is involved in many different aspects for this company such as research and development projects, analysis, inventory, billing, writing SOP's and facilitates/maintains communication between RPCI and ITN on a daily basis.
Dalin Pan Dalin Pan, M.S.
Dalin Pan got his masters degree on Pharmacology at State University of New York at Buffalo. He has five years of experience working in the Flow Cyotmetry Resource and eight years of experience performing molecular and biological research. He manages the laboratory supplies needed to maintain the research laboratory and performs experiments required by investigators who do not perform their own. This includes processing the specimens, analyzing the data, reviewing the data with the investigators, and providing final graphs and tables for publication.
Mike Rickard Michael Rickert, M.S.
Michael joined the Department of Flow Cytometry in 2004, and has worked at Roswell Park since 1979 in several laboratories. He received a Bachelor's degree in Biology from the University of Rochester and a Master's degree in Natural Sciences from SUNY at Buffalo. Currently he is responsible for tracking and improving departmental billing, accounts and purchases, having previously assayed and analyzed samples using the Luminex system.
Joe Tario Joe D. Tario, Jr.
Joseph is a PhD candidate in the Department of Cancer Pathology and is performing his dissertation work in this laboratory on the use of dendritic cell vaccines in the treatment of cancer. His diverse background in cellular biology and prior experience in flow cytometry have been valuable assets in the progression of his studies and also to the collective research group. Joseph is a diligent worker and evidently and ardent Neil Diamond enthusiast, as he can sometimes be overheard singing 'Forever in Blue Jeans' while running experiments into the late hours of the evening.
Orla Maguire Orla Maguire
Orla is a post-doc who joined the department in August 2010 to research the clinical applications of the ImageStream technology. She is involved in preparation of publications and grant applications, and also assists users with the ImageStream. Since her background is in molecular biology, she is drawing on the expertise of everyone here to become adept in all aspects of Flow Cytometry.
Ree Dolnick Ree Dolnick
Ree is a Flow Cytometry specialist who joined the Department of Flow Cytometry in 2009.  Her responsibilities include technical support and operational management of the Luminex, Live Cell Imaging, and Confocal Facilities. Educational background includes undergraduate and graduate degrees in molecular biology and pharmacology.
Craig Jones Craig Jones
Craig A. Jones received his BA in Cell and Molecular Biology from the University of Buffalo in 1977 after which he worked as a research technician in the Department of Microbiology, Univ. of Buf. This work involved examining issues related to the biochemistry and immunology of oral bacteria. During this time Craig gained experience in various types of immunoassays. He then attended graduate school where he obtained his MA in 1983 and Ph.D. in 1987 from the Department of Microbiology. He has worked at RPCI since that time in the laboratory of Dr. Kenneth Gross, Department of Molecular and Cellular Biology. Craig joined Flow Cytometry and Imaging in the summer of 2011 where he works on cell sorting and any other project assigned by Hans or Fearless Leader.
Paul Wallace Kitty de Jong Ph.D.
Kitty joined RPCI in 2012 and specializes in singing as well as operating Arias. She is part of the staff responsible for all cell sorting experiments, and also assists research users on the analysis equipment. Kitty started using flow cytometry in 1995 and has been a fan of the technique ever since. She has a PhD in biochemistry from the University of Bern in Switzerland (but she is Dutch) and worked for many years in research on red blood cells. She even has publications outlining new assays by flow cytometry, and has experience using various instruments and analysis software versions. She also worked for Miltenyi Biotec and is the go-to person for any questions on magnetic bead sorting techniques including operation of the AutoMACS.