FLOW CYTOMETRY INSTRUMENTATION
Our Research Flow Cytometry facility houses five Becton Dickinson flow analyzers (Calibur, LSRIIA, LSRIIB, Fortessa A, and Fortessa B) and a Zetaview small particle analyzer that are accessible 24/7 to all trained investigators. In addition, elsewhere in our facility there are several breadboard custom instruments, a Beckman Coulter Gallios, and two cell sorters that can be accessed together with flow staff during regular business hours (Monday – Friday, 8am – 6pm).
During weekdays, our staff performs start-up procedures and daily QC on the analyzers. During weekends and on holidays, users are responsible for these procedures. Investigators are expected to work in the facility independently, but staff is available during regular business hours for consultation. Our instrument room is designated a BSL2 facility, which means lab coats and gloves must be worn.
Users can sign up for time on the equipment using the FOM calendar. Unrestricted individual access to this system is granted only after completion of the free Introduction to Flow Cytometry class (sign up here). The class outlines the requirements for use of the facility, and gives a basic overview of the technology. Further one-on-one training to become proficient on the instruments is available by appointment with our staff.
Instrument use is charged by actual usage time or 80% of calendar reservation time, whichever is greater. Reservations can be altered up to two hours before the start time. Reduced charges apply during night time (8pm – 8am) and during weekends and holidays.
Custom Flow Cytometers
The Flow & Imaging Electronics Shop provides a unique service to the core facility. The modestly equipped shop has the ability to service, repair, modify or construct flow cytometers utilized by Roswell staff. Custom technology available for studies include contiguous multispectral detection using diffraction grating optics, Infra-red flow cytometry, fluorescence detection beyond 1100 nm and an optical breadboard flow cytometer open to diverse configurations. Researchers interested in using this technology should contact Ed Podniesinski (716-845-8775).