FACSCalibur

Technical Specifications

  • Dual laser, separate interrogation for each laser
  • 4 color detection + 2 scatter parameters (from 488nm) + Time
  • Pulse processed Area & Width for discriminating doublets
  • Hydrodynamically focused sample interrogated within a quartz cuvette flow cell
  • Push button fluidic control
  • Sample aspiration rates at 12ul, 35ul, 60ul per minute
  • 1024 channel dynamic range on all parameters
  • OS X version 10.6, CellQuest Pro version 6.0

Excitation Sources

  • 488nm 15mW primary laser, air cooled argon-ion
  • 635nm 10mW secondary diode laser

Emission Detected

  • 488nm laser intercept:
    • 488/10 Forward Scatter
    • 488/10 Side Scatter
    • 530/30, FL1, FITC
    • 585/42, FL2, PE
    • 670LP, FL3, PerCP
  • 635nm laser intercept:
    • 661/16, FL4, APC