FACSAria II

Technical Specifications

  • Quartz cuvette flow cell is gel-coupled by refractive index-matching optical gel to the fluorescent objective lens. Sort fractions collected jet-air
  • 4 laser
  • 15 parameters: 13 fluorescent and two scatter parameters + Time
  • Sheath pressure is adjustable from 2 to 75 psi.
  • At 70 psi and 90 kHz with an average threshold rate of 25,000 events per second for a four-way sort results in a purity of > 98% and a yield > 80% of Poisson's expected yield
  • Aerosol Management Option (AMO)
  • 262,144 channel (18 bit) dynamic range on all parameters
  • Windows XP-Pro OS, Diva software acquisition

Excitation Sources

  • 355nm Uniphase Xcyte 100nW
  • 405nm Coherent cube variable power thru software 100mW
  • 488nm Coherent® Sapphire™ solid state variable power thru software: 50-150mW
  • 640nm Coherent Cube variable power thru software 40mW

Emission Detected

  • 488nm laser interept:
    • 488/10 FSC
    • 488/10 SSC
    • 530/30nm FITC
    • 575/25nm PE
    • 610/20nm PE-Texas Red™®
    • 710/50nm PerCP -Cy5.5, PE-Cy5.55
    • 780/60nm PE-Cy™7
  • 640nm laser interept:
    • 660/20nm APC
    • 730/30nm Alexa-700
    • 780/60nm APC-Cy7
  • 405nm laser interept:
    • 450/50nm DAPI, Pacific Blue, BV421
    • 530/30nm AmCyan, BV510
    • 650nmLP Side Population
  • 355nm laser interept:
    • 450/50 DAPI, Hoechst
    • 670LP Side Population