FACSAria I

Technical Specifications

  • Quartz cuvette flow cell is gel-coupled by refractive index-matching optical gel to the fluorescent objective lens. Sort fractions collected jet-air
  • 4 Laser - separate interrogation of sample for each laser
  • 14 parameters: 12 fluorescent and 2 scatter parameters + Time
  • Sheath pressure is adjustable from 2 to 75 psi
  • At 70 psi and 90 kHz with an average threshold rate of 25,000 events per second for a four-way sort results in a purity of > 98% and a yield > 80% of Poisson's expected yield
  • Sample Collection Cooling 4°, 20°, 37°, and 42°C (Water recirculator for refrigeration/heating)
  • Aerosol Management Option (AMO) with Bio-Bubble negative pressure. Bio Safety Level 2+ environment
  • 262,144 channel (18 bit) dynamic range on all parameters, Windows XP-Pro OS, Diva software acquisition

Excitation Sources

  • 405nm, Point Source Violet solid state: 30 mW
  • 488nm, Coherent® Sapphire™ solid state: 20mw
  • 561nm, Coherent solid state: 35mW
  • 633nm, HeNe air-cooled: 20mW

Emission Detected

  • 405nm Violet Laser intercept:
    • 450/50nm Pac Blue, BV421
    • 530/30nm AmCyan BV510
  • 488nm Blue Laser intercept:
    • 488/10nm FSC
    • 488/10nm SSC
    • 530/30nm FITC/Alexa 488
    • 710/50nm PerCP, PerCP-Cy5.5
  • 561nm (Yellow/ Green) intercept:
    • 585/15nm PE, dsRed
    • 610/20nm PE-TxRd/mCherry
    • 670/30nm PE-Cy5
    • 710/50nm PE-Cy5.5
    • 780/60nm PE Cy7
  • 633nm Red Laser intercept:
    • 660/20nm APC
    • 730/30nm Alexa-700
    • 780/60nm APC-Cy7