Flow and Image Cytometry Applications
Each fall the Department of Flow and Image Cytometry Department offers a semester long, four credit hour, graduate course (MIR 524) focusing on different flow and image cytometry applications. An overriding goal of the course is to prepare individuals to comfortably sit at any of our cytometers and knowledgeably set it up and collect really good data. This course will review principles, theoretical background and applications of current flow and image cytometry techniques. This course is highly relevant to students in the biological and medical sciences. We focus on immunophenotyping from designing an experiment, progressing through staining techniques, data acquisition, and data analysis. Modules on DNA cell cycle, immune monitoring, cell signaling, sorting, confocal microscopy, ImageStream round out the course.
Course ObjectivesAt the end of this course, students are expected to:
- Describe the basic hardware components of a flow cytometer, imaging flow cytometer and a confocal microscope and their function
- Understand the experimental design and analysis principles of flow and image cytometry
- Become knowledgeable in cytometry applications including the study of cellular phenotype, cell proliferation, cell death, intra- and inter cellular signaling and functional assays
- Be able to apply the above theories and strategies in planning and critically evaluating flow and image cytometry studies
- Become familiar with clinical and research applications of flow and image cytometry
The course is limited in size and priority will be given to Roswell Park and UB graduate students. If you are interested in more information or would like to sign up, please contact Alexis Conway.