Cell Imaging

Confocal Microscopy

Our Leica TCS SP8 Laser Scanning Spectral Confocal Microscope was put in operation early 2018. The confocal has 4 laser lines (405nm, 488nm, 552nm, and 638nm) and is equipped with 10X dry, 20X dry, and 63X oil immersion objectives. Further specifications are detailed in the menu on the right.

First-time users of the Leica TCS SP8 confocal microscope must contact the principal operator Carlos Cedeno (716-845-8235) to schedule a new user training session (~45 minutes) before working independently on the microscope. All users should reserve time on the microscope using the FOM calendar system. Operator assistance is available by scheduling an appointment during regular business hours.

Live Cell Imager

The Leica AF6000LX live cell imaging system enables time-lapse videography of cell cultures in a temperature- and CO2-controlled environment. The microscope stage is adaptable to receiving multi-well plate formats as well as slide based formats (including multi-well chamber slides). The software enables multiple user-defined imaging positions as well as user-defined imaging time intervals. In addition to polarized bright field imaging, the instrument has B/G/R fluorescent imaging capabilities using a mercury short-arc lamp excitation source. For multiple position imaging the microscope is equipped with long-working distance 10x, 20x and 40x objectives. A 63x objective is also available but due to the requirement of immersion-oil, this magnification is not suitable for multiple positions if the positions require relatively large stage movement or for long time periods of imaging.

The Live Cell Imager is available by appointment only. To make an appointment please contact Hans Minderman (716-845-1162) or Carlos Cedeno (716-845-8235).