Our New Leica TCS SP8 Confocal Platform

From live cell to quantitative imaging, from super-sensitivity to super-resolution, from multiphoton to light sheet imaging – with the Leica TCS SP8 Confocal platform one can always expand their possibilities. It is built on a modular concept: One can tailor this microscope to their current needs and upgrade additional functionalities at any time.

In confocal microscopy, the excitation light enters the specimen on the same side from which emission is collected. A beam splitter separates excitation and emission light. The AOBS (Acousto-Optical Beam Splitter) is a completely transparent, active TeO2 crystal, which offers maximum photon efficiency. An acoustic wave coupled into the crystal changes its transmission properties. It switches within microseconds by simply changing the radio frequency of the wave. This makes the AOBS a uniquely flexible, efficient, and fast beam splitter.

The AOBS reflection bands have a steep cut-off and narrow bandwidth allowing the collection of up to 30% more emission light. The AOBS can accomodate up to eight reflection bands. You can use closely spaced excitation lines for simultaneous imaging of unique dye combinations like GFP and YFP. As a result, dye separation can be easily performed without unmixing and related artifacts.